Proteomics Reveals the Mechanism Underlying the Inhibition of Phytophthora sojae by Propyl Gallate.
Identifieur interne : 000080 ( Main/Exploration ); précédent : 000079; suivant : 000081Proteomics Reveals the Mechanism Underlying the Inhibition of Phytophthora sojae by Propyl Gallate.
Auteurs : Dong Liu [République populaire de Chine] ; Yuemin Pan [République populaire de Chine] ; Kunyuan Li [République populaire de Chine] ; Dandan Li [République populaire de Chine] ; Ping Li [République populaire de Chine] ; Zhimou Gao [République populaire de Chine]Source :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2020.
Abstract
Phytophthora sojae is a serious soil-borne pathogen, and the major control measures undertaken include the induction of soybean-resistance genes, fungicides, and scientific and reasonable planting management. Owing to the safety and resistance of fungicides, it is of great importance to screen new control alternatives. In a preliminary study, we observed that propyl gallate (PG) exerts a considerable inhibitory effect on P. sojae and can effectively prevent and cure soybean diseases, although the underlying mechanism remains unclear. To explore the inhibitory mechanism of PG on P. sojae, we analyzed the differences in the protein profile of P. sojae before and after treatment with PG using tandem mass tag (TMT) proteomics. Proteomic analysis revealed that the number of differentially expressed proteins (DEPs) was 285, of which 75 were upregulated and 210 were downregulated, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways primarily comprised glycolysis, tricarboxylic acid cycle, fatty acid metabolism, secondary metabolite generation, and other pathways. Among the DEPs involved in PG inhibition of P. sojae are two closely related uncharacterized proteins encoded by PHYSODRAFT_522340 and PHYSODRAFT_344464, denoted PsFACL and PsCPT herein. The CRISPR/Cas9 knockout technique revealed that PsFACL and PsCPT were involved in the growth rate and pathogenicity. In addition, the results of gas chromatography-mass spectrometry (GC-MS) showed that there were differences in fatty acid levels between wild-type (WT) and CRISPR/Cas9 knockout transformants. Knocking out PsFACL and PsCPT resulted in the restriction of the synthesis and β-oxidation of long-chain fatty acids, respectively. These suggest that PsFACL and PsCPT were also involved in the regulation of the fatty acid metabolism. Our results aid in understanding the mechanism underlying the inhibition of P. sojae growth by PG.
DOI: 10.1021/acs.jafc.0c02371
PubMed: 32633954
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first="Yuemin" last="Pan">Yuemin Pan</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Anhui Agricultural University, 130 West of Changjiang Road, Hefei 230036, Anhui, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Anhui Agricultural University, 130 West of Changjiang Road, Hefei 230036, Anhui</wicri:regionArea><wicri:noRegion>Anhui</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei 230036, Anhui, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei 230036, Anhui</wicri:regionArea><wicri:noRegion>Anhui</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key 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Chine</country><wicri:regionArea>Department of Horticulture and Landscape, Anqing Vocational and Technical College, 99 North of Tianzhushan Road, Anqing 246003, Anhui</wicri:regionArea><wicri:noRegion>Anhui</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Zhimou" sort="Gao, Zhimou" uniqKey="Gao Z" first="Zhimou" last="Gao">Zhimou Gao</name><affiliation wicri:level="1"><nlm:affiliation>College of Plant Protection, Anhui Agricultural University, 130 West of Changjiang Road, Hefei 230036, Anhui, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Plant Protection, Anhui Agricultural University, 130 West of Changjiang Road, Hefei 230036, Anhui</wicri:regionArea><wicri:noRegion>Anhui</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Phytophthora sojae</i> is a serious soil-borne pathogen, and the major control measures undertaken include the induction of soybean-resistance genes, fungicides, and scientific and reasonable planting management. Owing to the safety and resistance of fungicides, it is of great importance to screen new control alternatives. In a preliminary study, we observed that propyl gallate (PG) exerts a considerable inhibitory effect on <i>P. sojae</i> and can effectively prevent and cure soybean diseases, although the underlying mechanism remains unclear. To explore the inhibitory mechanism of PG on <i>P. sojae</i>, we analyzed the differences in the protein profile of <i>P. sojae</i> before and after treatment with PG using tandem mass tag (TMT) proteomics. Proteomic analysis revealed that the number of differentially expressed proteins (DEPs) was 285, of which 75 were upregulated and 210 were downregulated, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways primarily comprised glycolysis, tricarboxylic acid cycle, fatty acid metabolism, secondary metabolite generation, and other pathways. Among the DEPs involved in PG inhibition of <i>P. sojae</i> are two closely related uncharacterized proteins encoded by <i>PHYSODRAFT_522340</i> and <i>PHYSODRAFT_344464</i>, denoted <i>PsFACL</i> and <i>PsCPT</i> herein. The CRISPR/Cas9 knockout technique revealed that <i>PsFACL</i> and <i>PsCPT</i> were involved in the growth rate and pathogenicity. In addition, the results of gas chromatography-mass spectrometry (GC-MS) showed that there were differences in fatty acid levels between wild-type (WT) and CRISPR/Cas9 knockout transformants. Knocking out <i>PsFACL</i> and <i>PsCPT</i> resulted in the restriction of the synthesis and β-oxidation of long-chain fatty acids, respectively. These suggest that <i>PsFACL</i> and <i>PsCPT</i> were also involved in the regulation of the fatty acid metabolism. Our results aid in understanding the mechanism underlying the inhibition of <i>P. sojae</i> growth by PG.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32633954</PMID><DateRevised><Year>2020</Year><Month>08</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5118</ISSN><JournalIssue CitedMedium="Internet"><Volume>68</Volume><Issue>31</Issue><PubDate><Year>2020</Year><Month>Aug</Month><Day>05</Day></PubDate></JournalIssue><Title>Journal of agricultural and food chemistry</Title><ISOAbbreviation>J Agric Food Chem</ISOAbbreviation></Journal><ArticleTitle>Proteomics Reveals the Mechanism Underlying the Inhibition of <i>Phytophthora sojae</i> by Propyl Gallate.</ArticleTitle><Pagination><MedlinePgn>8151-8162</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.jafc.0c02371</ELocationID><Abstract><AbstractText><i>Phytophthora sojae</i> is a serious soil-borne pathogen, and the major control measures undertaken include the induction of soybean-resistance genes, fungicides, and scientific and reasonable planting management. Owing to the safety and resistance of fungicides, it is of great importance to screen new control alternatives. In a preliminary study, we observed that propyl gallate (PG) exerts a considerable inhibitory effect on <i>P. sojae</i> and can effectively prevent and cure soybean diseases, although the underlying mechanism remains unclear. To explore the inhibitory mechanism of PG on <i>P. sojae</i>, we analyzed the differences in the protein profile of <i>P. sojae</i> before and after treatment with PG using tandem mass tag (TMT) proteomics. Proteomic analysis revealed that the number of differentially expressed proteins (DEPs) was 285, of which 75 were upregulated and 210 were downregulated, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways primarily comprised glycolysis, tricarboxylic acid cycle, fatty acid metabolism, secondary metabolite generation, and other pathways. Among the DEPs involved in PG inhibition of <i>P. sojae</i> are two closely related uncharacterized proteins encoded by <i>PHYSODRAFT_522340</i> and <i>PHYSODRAFT_344464</i>, denoted <i>PsFACL</i> and <i>PsCPT</i> herein. The CRISPR/Cas9 knockout technique revealed that <i>PsFACL</i> and <i>PsCPT</i> were involved in the growth rate and pathogenicity. In addition, the results of gas chromatography-mass spectrometry (GC-MS) showed that there were differences in fatty acid levels between wild-type (WT) and CRISPR/Cas9 knockout transformants. Knocking out <i>PsFACL</i> and <i>PsCPT</i> resulted in the restriction of the synthesis and β-oxidation of long-chain fatty acids, respectively. These suggest that <i>PsFACL</i> and <i>PsCPT</i> were also involved in the regulation of the fatty acid metabolism. Our results aid in understanding the mechanism underlying the inhibition of <i>P. sojae</i> growth by PG.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Dong</ForeName><Initials>D</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-5846-3196</Identifier><AffiliationInfo><Affiliation>College of Plant Protection, Anhui Agricultural University, 130 West of Changjiang Road, Hefei 230036, Anhui, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Horticulture and Landscape, Anqing Vocational and Technical College, 99 North of Tianzhushan Road, Anqing 246003, Anhui, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Yuemin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Anhui Agricultural University, 130 West of Changjiang Road, Hefei 230036, Anhui, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Anhui Province Key Laboratory of Integrated Pest Management on Crops, Anhui Agricultural University, Hefei 230036, Anhui, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, Anhui Agricultural University, Anqing 246003, Anhui, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Kunyuan</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Anhui Agricultural University, 130 West of Changjiang Road, Hefei 230036, Anhui, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Dandan</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Anhui Agricultural University, 130 West of Changjiang Road, Hefei 230036, Anhui, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Ping</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Horticulture and Landscape, Anqing Vocational and Technical College, 99 North of Tianzhushan Road, Anqing 246003, Anhui, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Zhimou</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Anhui Agricultural University, 130 West of Changjiang Road, Hefei 230036, Anhui, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>07</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Agric Food Chem</MedlineTA><NlmUniqueID>0374755</NlmUniqueID><ISSNLinking>0021-8561</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">CRISPR/Cas9</Keyword><Keyword MajorTopicYN="N">Phytophthora sojae</Keyword><Keyword MajorTopicYN="N">inhibitory mechanism</Keyword><Keyword MajorTopicYN="N">propyl gallate</Keyword><Keyword MajorTopicYN="N">proteomics</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32633954</ArticleId><ArticleId IdType="doi">10.1021/acs.jafc.0c02371</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Dong" sort="Liu, Dong" uniqKey="Liu D" first="Dong" last="Liu">Dong Liu</name></noRegion><name sortKey="Gao, Zhimou" sort="Gao, Zhimou" uniqKey="Gao Z" first="Zhimou" last="Gao">Zhimou Gao</name><name sortKey="Li, Dandan" sort="Li, Dandan" uniqKey="Li D" first="Dandan" last="Li">Dandan Li</name><name sortKey="Li, Kunyuan" sort="Li, Kunyuan" uniqKey="Li K" first="Kunyuan" last="Li">Kunyuan Li</name><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name><name sortKey="Liu, Dong" sort="Liu, Dong" uniqKey="Liu D" first="Dong" last="Liu">Dong Liu</name><name sortKey="Pan, Yuemin" sort="Pan, Yuemin" uniqKey="Pan Y" first="Yuemin" last="Pan">Yuemin Pan</name><name sortKey="Pan, Yuemin" sort="Pan, Yuemin" uniqKey="Pan Y" first="Yuemin" last="Pan">Yuemin Pan</name><name sortKey="Pan, Yuemin" sort="Pan, Yuemin" uniqKey="Pan Y" first="Yuemin" last="Pan">Yuemin Pan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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